At present, there are various expression systems available for the production of biological products such as vaccines, including primary cells, diploid cells, and passage cells from various animals.
In the 1960s, the role of human diploid cells was discovered and rapidly used to combat destructive infectious pathogens, including measles, mumps, rubella, poliomyelitis, hepatitis A, and rabies virus, and has since played a key role in vaccine development.
To assist vaccine manufacturers in achieving expected productivity through the use of diploid cells, while reducing production costs and serum usage, and overcoming the challenges posed by medium adaptation in cell culture.
Thermo Fisher Scientific launches Gibco ™ Diploid growth low serum medium (SRM) system. It includes two parts: low serum medium (SRM) for cell growth and serum-free medium (SFM) for cell toxicity. Human diploid cells, including MRC-5 (item number: CCL-171), WI-38 (item number: CCL-75), KMB-17, and 2BS, as well as chicken embryo fibroblasts (CEF), can be used in this system* The above cells can be imported from our company as ATCC. Some viruses can be imported
Product features:
1. Human diploid cells (such as MRC-5, WI-38, and KMB17) can grow in a medium containing only 1-2% serum;
2. Support the growth of chicken embryo fibroblasts under serum-free conditions;
3. Virus production can be cahhied out without adding albumin or other supplements under serum-free conditions;
4. It can be directly used for cell revival without adaptation.
At present, we have confirmed that diploid culture medium has been successfully used for chickenpox, vesicular stomatitis virus, mumps, enterovirus, hepatitis A virus, etc., and its potency is not lower than traditional culture medium, and in some cases, it is one log value higher than traditional culture medium.
give an example 1
Application of diploid culture medium (1% FBS) in large-scale cultivation and serum-free toxin production of MRC-5 cells.
Cell line: MRC-5 inoculation density 1.5 * 10 ^ 4cell/cm2
DSFM toxin producing culture medium item number: A3969001
DSRM low serum growth medium item number: A3968901
Serum: GIBCO fetal bovine serum, item number: 10099141C (from Australia); Item number: 10091148 (New Zealand origin)
Virus: vesicular stomatitis virus
give an example 2
Application of diploid culture medium in the cultivation of 2BS cells and production of hepatitis A virus.
Cell: 2BS
Virus: Hepatitis A virus
Toxic production: DSFM serum-free
Growth: DSRM low serum+2% serum
As shown in the figure, diploid culture medium has a significant amplification effect on low serum culture and serum-free toxin production of 2BS cells.
give an example 3
The toxin production results of diploid culture medium in CEF fibroblast culture and rubella vaccine.
Cell: CEF
Toxic production: DSFM serum-free
Growth: DSRM low serum
As shown in the figure, CEF chicken embryo fibroblasts were cultured in serum-free medium during the early digestion process, and after serum-free digestion with TrypLE trypsin, they were virus-free. The control group was cultured in serum-free medium after digestion was terminated, and the final toxin production titers were comparable, achieving a process transition from serum-free to serum-free.
In summary, Gibco diploid medium is a suitable medium for low serum large-scale cultivation and efficient toxin production without serum. Diploid medium can be directly used for cell recovery without the need for adaptation.
Especially in the context of tight global serum supply, vaccine companies can reduce their dependence on serum, lower production and purification costs, and improve product quality consistency and safety.
Product ordering information
产品货号 | 产品描述 |
A3968902 | Diploid Growth Medium Kit， 100L |
A3968901 | Diploid Growth Medium Kit， 10L |
A3969002 | Diploid Production Medium Kit， 100L |
A3969001 | Diploid Production Medium Kit， 10L |
10099141C | 澳洲胎牛血清 |
10091148 | 新西兰胎牛血清 |
12563029 | TrypLE™ Select 酶 (1X)，无酚红 500mL |
A1217702 | TrypLE™ Select 酶 (10X)，无酚红 500mL |